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KMID : 0881720160310060465
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Journal of Food Hygiene and Safety
2016 Volume.31 No. 6 p.465 ~ p.470
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Effects of Sample Preparation Methods for the Isolation of Foodborne Pathogens from Sprout Seeds
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Kim Won-Il
Kim Sun-Young
Kim In-Seon
Han Sang-hyun
Kim Se-Ri
Yun Bo-hyun
Ryu Jae-Gee
Kim Hyun-Ju
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Abstract
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Sample preparation methods were evaluated for effectiveness in detecting foodborne pathogens from sprout seeds. The methods included: Rinse.-Test portions were rinsed with 0.1% peptone water, and the pellet after centrifugation was inoculated into pre-enrichment media; and Sprouting.-Seed samples were sprouted before pre-enrichment and sprouted seeds were inoculated into pre-enrichment media. In rinse method, E. coli was isolated from 13 of 280 sample units. In sprouting method, E. coli was isolated from 12 of 135 sample units. E. coli O157:H7, Salmonella spp., and L. monocytogenes were not detected in any of the samples. In the trials for recovering Salmonella enterica from artificially contaminated alfalfa seeds, the soak, rinse, and sprouting methods were evaluated. The detection rates of S. enterica were statistically different according to the amount of the sample tested and selective medium type (P < 0.05).
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KEYWORD
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sprout seeds, foodborne pathogens, sample preparation method
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